| 质粒名称: | pGL3 1 kb prom and exon1 fragment CD274 |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 107006 |
| 存储实验室: | Julian Downward |
|
载体骨架基本信息 |
|
| 载体名称: | pGL3 |
| 载体出品公司: | Promega |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | -- |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | 哺乳动物表达，荧光素酶 |
| 筛选标记: | -- |
| 高拷贝/低拷贝: | -- |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | CD274,PD-L1, B7-H1 |
| 物种来源: | H. sapiens (human) |
| 插入大小: | 1134 bp |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子 | -- |
| 标签/融合蛋白: | -- |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | MluI（销毁后未知） |
| 3' 克隆位点: | XhoI（销毁后未知） |
| 5' 测序引物 | RVprimer3 |
| 3' 测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | pGL3 1 kb prom and exon1 fragment CD274 was a gift from Julian Downward (Addgene plasmid # 107006 ; http://n2t.net/addgene:107006 ; RRID:Addgene_107006) |
| 参考文献部分: | Oncogenic RAS Signaling Promotes Tumor Immunoresistance by Stabilizing PD-L1 mRNA. Coelho MA, de Carne Trecesson S, Rana S, Zecchin D, Moore C, Molina-Arcas M, East P, Spencer-Dene B, Nye E, Barnouin K, Snijders AP, Lai WS, Blackshear PJ, Downward J. Immunity. 2017 Dec 19;47(6):1083-1099.e6. doi: 10.1016/j.immuni.2017.11.016. Epub 2017 Dec 12. 10.1016/j.immuni.2017.11.016 PubMed 29246442 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pGL3 1 kb prom and exon1 fragment CD274 5941 bp ds-DNA circular SYN 06-AUG-2018
DEFINITION Modified Luciferase expression vector.
ACCESSION .
VERSION .
KEYWORDS pGL3 1 kb prom and exon1 fragment CD274
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 5941)
AUTHORS Coelho MA, de Carne Trecesson S, Rana S, Zecchin D, Moore C,
Molina-Arcas M, East P, Spencer-Dene B, Nye E, Barnouin K, Snijders
AP, Lai WS, Blackshear PJ, Downward J
TITLE Oncogenic RAS Signaling Promotes Tumor Immunoresistance by
Stabilizing PD-L1 mRNA.
JOURNAL Immunity. 2017 Dec 19;47(6):1083-1099.e6. doi:
10.1016/j.immuni.2017.11.016. Epub 2017 Dec 12.
PUBMED 29246442
REFERENCE 2 (bases 1 to 5941)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 6, 2018 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..5941
/organism="synthetic DNA construct"
/mol_type="other DNA"
polyA_signal 7..55
/note="synthetic polyadenylation signal"
misc_feature 69..160
/label=pause site
/note="RNA polymerase II transcriptional pause signal from
the human alpha-2 globin gene"
primer_bind 109..128
/label=RVprimer3
/note="pGL3 vector, forward primer"
regulatory 1372..1381
/regulatory_class="other"
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
CDS 1378..3030
/codon_start=1
/gene="luc+"
/product="firefly luciferase"
/label=luciferase
/note="enhanced luc+ version of the luciferase gene"
/translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
primer_bind complement(1444..1462)
/label=LucNrev
/note="Firefly luciferase, reverse primer"
polyA_signal 3071..3192
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(3082..3101)
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
primer_bind 3136..3155
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind complement(3440..3457)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(3611..4199)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(3691..3710)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(4370..5230)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 4993..5012
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(5231..5335)
/gene="bla"
/label=AmpR promoter
rep_origin 5362..5817
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(5449..5468)
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 5659..5680
/label=F1ori-F
/note="F1 origin, forward primer"
ORIGIN
1 ggccgcaata aaatatcttt attttcatta catctgtgtg ttggtttttt gtgtgaatcg
61 atagtactaa catacgctct ccatcaaaac aaaacgaaac aaaacaaact agcaaaatag
121 gctgtcccca gtgcaagtgc aggtgccaga acatttctct atcgataggt accgagctct
181 tacgcgtgct ttaatcttcg aaactcttcc cggtgaaaat ctcatttaca agaaaactgg
241 actgacatgt ttcactttct gtttcatttc tatacacagc tttattccta ggacaccaac
301 actagatacc taaactgaaa gcttccgccg atttcaccga aggtcaggaa agtccaacgc
361 ccggcaaact ggatttgctg ccttgggcag aggtgggcgg gaccccgcct ccgggcctgg
421 cgcaacgctg agcagctggc gcgtcccgcg cggccccagt tctgcgcagc ttcccgaggc
481 tccgcaccag ccgcgcttct gtccgcctgc aggtagggag cgttgttcct ccgcgggtgc
541 ccacggccca gtatctctgg ctagctcgct gggcacttta ggacggaggg tctctacacc
601 ctttctttgg gatggagaga ggagaaggga aagggaacgc gatggtctag ggggcagtag
661 agccaattac ctgttggggt taataagaac aggcaatgca tctggccttc ctccaggcgc
721 gattcagttt tgctctaaaa ataatttata cctctaaaaa taaataagat aggtagtata
781 ggataggtag tcattcttat gcgactgtgt gttcagaata tagctctgat gctaggctgg
841 aggtctggac acgggtccaa gtccaccgcc agctgcttgc tagtaacatg acttgtgtaa
901 gttatcccag ctgcagcatc taagtaagtc tcttcctgcg ctaagcaggt ccaggatccc
961 tgaacggaat ttatttgctc tgtccattct gagaacccaa aggagtccta aaagaggaat
1021 ggaggagcct aagaataaaa atagtataat aaaacatttc ttagacacat tgaccttggc
1081 ctatgtcaaa gttcagtctg ggtttgtctt ataacacaag gagtaaaagt accattgttc
1141 tacctctttt tttaatactt gaaaaaaatt tactgtggat gcttttctat gaattaaata
1201 accttctaaa aaatgttttc attgctgcat tcgattagat tgggtaacta aatgaaatta
1261 attcctcact gttgggtata aaggttattt acagtggttc tgtcttagcc attcactgaa
1321 cctcgagatc tgcgatctaa gtaagcttgg cattccggta ctgttggtaa agccaccatg
1381 gaagacgcca aaaacataaa gaaaggcccg gcgccattct atccgctgga agatggaacc
1441 gctggagagc aactgcataa ggctatgaag agatacgccc tggttcctgg aacaattgct
1501 tttacagatg cacatatcga ggtggacatc acttacgctg agtacttcga aatgtccgtt
1561 cggttggcag aagctatgaa acgatatggg ctgaatacaa atcacagaat cgtcgtatgc
1621 agtgaaaact ctcttcaatt ctttatgccg gtgttgggcg cgttatttat cggagttgca
1681 gttgcgcccg cgaacgacat ttataatgaa cgtgaattgc tcaacagtat gggcatttcg
1741 cagcctaccg tggtgttcgt ttccaaaaag gggttgcaaa aaattttgaa cgtgcaaaaa
1801 aagctcccaa tcatccaaaa aattattatc atggattcta aaacggatta ccagggattt
1861 cagtcgatgt acacgttcgt cacatctcat ctacctcccg gttttaatga atacgatttt
1921 gtgccagagt ccttcgatag ggacaagaca attgcactga tcatgaactc ctctggatct
1981 actggtctgc ctaaaggtgt cgctctgcct catagaactg cctgcgtgag attctcgcat
2041 gccagagatc ctatttttgg caatcaaatc attccggata ctgcgatttt aagtgttgtt
2101 ccattccatc acggttttgg aatgtttact acactcggat atttgatatg tggatttcga
2161 gtcgtcttaa tgtatagatt tgaagaagag ctgtttctga ggagccttca ggattacaag
2221 attcaaagtg cgctgctggt gccaacccta ttctccttct tcgccaaaag cactctgatt
2281 gacaaatacg atttatctaa tttacacgaa attgcttctg gtggcgctcc cctctctaag
2341 gaagtcgggg aagcggttgc caagaggttc catctgccag gtatcaggca aggatatggg
2401 ctcactgaga ctacatcagc tattctgatt acacccgagg gggatgataa accgggcgcg
2461 gtcggtaaag ttgttccatt ttttgaagcg aaggttgtgg atctggatac cgggaaaacg
2521 ctgggcgtta atcaaagagg cgaactgtgt gtgagaggtc ctatgattat gtccggttat
2581 gtaaacaatc cggaagcgac caacgccttg attgacaagg atggatggct acattctgga
2641 gacatagctt actgggacga agacgaacac ttcttcatcg ttgaccgcct gaagtctctg
2701 attaagtaca aaggctatca ggtggctccc gctgaattgg aatccatctt gctccaacac
2761 cccaacatct tcgacgcagg tgtcgcaggt cttcccgacg atgacgccgg tgaacttccc
2821 gccgccgttg ttgttttgga gcacggaaag acgatgacgg aaaaagagat cgtggattac
2881 gtcgccagtc aagtaacaac cgcgaaaaag ttgcgcggag gagttgtgtt tgtggacgaa
2941 gtaccgaaag gtcttaccgg aaaactcgac gcaagaaaaa tcagagagat cctcataaag
3001 gccaagaagg gcggaaagat cgccgtgtaa ttctagagtc ggggcggccg gccgcttcga
3061 gcagacatga taagatacat tgatgagttt ggacaaacca caactagaat gcagtgaaaa
3121 aaatgcttta tttgtgaaat ttgtgatgct attgctttat ttgtaaccat tataagctgc
3181 aataaacaag ttaacaacaa caattgcatt cattttatgt ttcaggttca gggggaggtg
3241 tgggaggttt tttaaagcaa gtaaaacctc tacaaatgtg gtaaaatcga taaggatccg
3301 tcgaccgatg cccttgagag ccttcaaccc agtcagctcc ttccggtggg cgcggggcat
3361 gactatcgtc gccgcactta tgactgtctt ctttatcatg caactcgtag gacaggtgcc
3421 ggcagcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc
3481 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg
3541 caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt
3601 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa
3661 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct
3721 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc
3781 cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg
3841 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct
3901 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag
3961 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga
4021 agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga
4081 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg
4141 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag
4201 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag
4261 ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat
4321 gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct
4381 taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac
4441 tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa
4501 tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg
4561 gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt
4621 gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca
4681 ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt
4741 cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct
4801 tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg
4861 cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg
4921 agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg
4981 cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa
5041 aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt
5101 aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt
5161 gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt
5221 gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca
5281 tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat
5341 ttccccgaaa agtgccacct gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg
5401 tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt
5461 tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc
5521 tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg
5581 gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg
5641 agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct
5701 cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg
5761 agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgctt acaatttgcc
5821 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
5881 tacgccagcc caagctacca tgataagtaa gtaatattaa ggtacgggag gtacttggag
5941 c
//
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